A simple, sensitive, rapid and reproducible reversed-phase HPLC methods has been developed and validated for simultaneous determination of cefixime trihydrate and levoflaxacin hemihydrate in pharmaceutical formulation. The HPLC analysis was performed on the C-8, 15 cm, 4.6 mm and 5 micron (waters) column, at ambient temperature using phosphate buffer: acetonitrile (70:30) as mobile phase and pH adjusted to 8.0 with triethyl amine. The flow rate was adjusted to 0.8 ml/min. The detection was carried out at 283 nm. Linearity was found to be in concentration range of 10-50 µg/ml for CEFI and LEVO with coefficient of correlation 0.999 and 0.999 respectively. LOD was found to be 0.00075 and 0.0002 for CEFI and LEVO respectively and LOQ was found to be 0.0022 and 0.059 for CEFI and LEVO respectively. Due to these attributes, the proposed method could be used for routine quality control analysis of these drugs in combined dosage forms. The method was validated as per ICH guidelines.
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